英文名稱    DFFB    

中文名稱    DNA斷裂因子抗體（蛋白酶激活脫氧核糖核酸酶）    

別    名    CAD; Caldesmon; Caspase activated deoxyribonuclease; Caspase activated DNase; Caspase activated nuclease; CDM; CPAN; Deoxyribonuclease III, caspase activated; DFF 40; DFF2; DFF40; Didff; DNA fragmentation factor 40 kDa subunit; DNA fragmentation factor subunit beta; DNA fragmentation factor, 40 Da, beta polypeptide (caspase activated DNase); DNA fragmentation factor, 40 kD beta subunit; DNA fragmentation factor, 40 kD, beta polypeptide; DNA fragmentation factor, 40kDa, beta polypeptide (caspase activated DNase); DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase); DNAation factor, beta subunit; DFFB_HUMAN.    

供 應 商    遠慕生物

研究領域    細胞生物  細胞凋亡  細胞周期蛋白  激酶和磷酸酶  表觀遺傳學      

抗體來源    Rabbit    

克隆類型    Polyclonal    

交叉反應    Human, Mouse, Rat, Dog, Rabbit,     

產品應用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 DNA斷裂因子抗體（蛋白酶激活脫氧核糖核酸酶）（石蠟切片需做抗原修復） 
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    39kDa    

細胞定位    細胞核 細胞漿     

性    狀    Lyophilized or Liquid    

濃    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human DFFB    

亞    型    IgG    

純化方法    affinity purified by Protein A    

儲 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

DNA斷裂因子抗體（蛋白酶激活脫氧核糖核酸酶）產品介紹    background:

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. [provided by RefSeq, Jul 2008].

Function:
Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. 

Subunit:
Heterodimer of DFFA and DFFB. Interacts with HIST1H1A. 

Subcellular Location:
Cytoplasm. Nucleus.

Similarity:
Contains 1 CIDE-N domain.