英文名稱 Dnmt3a

中文名稱 DNA甲基轉移酶-3α抗體

別    名 DNA (cytosine 5 ) methyltransferase 3 alpha; DNA (cytosine 5) methyltransferase 3A; DNA cytosine methyltransferase 3A2; DNA methyltransferase 3a; DNA methyltransferase HsaIIIA; DNA MTase HsaI; DNA MTase HsaIIIA; DNMT 3a; DNMT; Dnmt3a; DNMT3A2; M HsaIIIA; M.HsaIIIA; MCMT; Methyl CpG binding domain protein 3a

供應商 遠慕生物

研究領域 腫瘤  細胞生物  信號轉導  細胞凋亡  表觀遺傳學  

抗體來源 Rabbit

克隆類型 Polyclonal

交叉反應 Human, Mouse, Rat, Cow, 

產品應用 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 Flow-Cyt=1ug/Test IF=1:100-500 （石蠟切片需做抗原修復） 

not yet tested in other applications.

optimal dilutions/concentrations should be determined by the end user.

分 子 量 100kDa

細胞定位 細胞核 細胞漿 

性    狀 Lyophilized or Liquid

濃    度 1mg/ml

免 疫 原  KLH conjugated synthetic peptide derived from human Dnmt3a:26-100/912 

亞    型 IgG

純化方法 affinity purified by Protein A

儲 存 液 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

保存條件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.

PubMedPubMed

產品介紹 background:

The tumor organization has the DNA methylation disorder, including and damps cancer gene high methylation DNA with the cell multiplication cycle close related cancer gene low methylation the methyl transferase (Dnmt) participation methylation the formation (is mainly Dnmt3a and Dnmt3b) and the maintenance (is mainly Dnmt1) (isoform CRA_b) (DNA methyltransferase MmuIIIA) (DNA MTase MmuIIIA)

Function:

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.

Subunit:

Heterotetramer composed of 1 DNMT3A homodimer and 2 DNMT3L subunits (DNMT3L-DNMT3A-DNMT3A-DNMT3L). Interacts with UBC9, PIAS1 and PIAS2. Binds the ZNF238 transcriptional repressor. Interacts with SETDB1. Associates with HDAC1 through its ADD domain. Interacts with DNMT1 and DNMT3B. Interacts with the PRC2/EED-EZH2 complex. Interacts with MPHOSPH8. Interacts with histone H3 that is not methylated at 'Lys-4' (H3K4).

Subcellular Location:

Nucleus. Cytoplasm. Note=Accumulates in the major satellite repeats at pericentric heterochromatin.

Tissue Specificity:

Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

Post-translational modifications:

Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.

Similarity:

Belongs to the C5-methyltransferase family. 

Contains 1 ADD domain. 

Contains 1 GATA-type zinc finger. 

Contains 1 PHD-type zinc finger. 

Contains 1 PWWP domain.

SWISS:

Q9Y6K1

Gene ID:

1788

Database links:

Entrez Gene: 1788 Human

Entrez Gene: 13435 Mouse

Omim: 602769 Human

SwissProt: Q9Y6K1 Human

SwissProt: O88508 Mouse

Unigene: 515840 Human

Unigene: 5001 Mouse

Important Note:

This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 

腫瘤組織存在DNA甲基化紊亂，包括與細胞增殖周期密切相關的癌基因低甲基化和抑癌基因高甲基化DNA甲基轉移酶(Dnmt)參與甲基化的形成(主要是Dnmt3a和Dnmt3b)和維持（主要是Dnmt1）