中文名稱    膠質細胞谷氨酸運載蛋白4抗體    

英文名稱    EAAT4  

別    名    Excitatory amino acid transporter 4; High affinity neuronal glutamate transporter; MGC33092; MGC43671; Sodium dependent glutamate/aspartate transporter; Solute carrier family 1 (high affinity aspartate/glutamate transporter) member 6; Solute carrier family 1 member 6;    

供 應 商    遠慕生物   

研究領域    細胞生物  染色質和核信號  轉錄調節因子  轉運蛋白      

抗體來源    Rabbit    

克隆類型    Polyclonal    

交叉反應    Human, Mouse, Rat, Dog, Pig, Cow, Sheep,     

產品應用    WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 Flow-Cyt=1:100-500 ICC=1:100-500 IF=1:100-500 膠質細胞谷氨酸運載蛋白4抗體（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    62kDa    

細胞定位    細胞膜     

性    狀    Lyophilized or Liquid    

濃    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human EAAT4    

亞    型    IgG    

純化方法    affinity purified by Protein A    

儲 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

膠質細胞谷氨酸運載蛋白4抗體產品介紹    background:

Excitatory Amino Acid Transporters (EAATs) are membrane-bound proteins that are localized in glial cells and pre-synaptic glutamatergic nerve endings. EAATs transport the excitatory neurotransmitters L-glutamate and D-aspartate, a process that is essential for terminating the postsynaptic action of glutamate. The re-uptake of amino acid neurotransmitters by EAAT proteins has been shown to protect neurons from excitotoxicity, which is caused by the accumulation of amino acid neurotransmitters. EAAT4 is an aspartate/glutamate transporter that is expressed predominantly in the cerebellum. The transport activity encoded by EAAT4 has high apparent affinity for L-aspartate and L-glutamate, and has a pharmacologic profile consistent with previously described cerebellar transport activities. EAAT5 is a glutamate transporter coupled to a chloride conductance which is expressed primarily in retina. Although EAAT5 shares the structural homologies of the EAAT family, a novel feature of the EAAT5 sequence is a carboxy-terminal motif previously identified in N-ethyl-D-aspartate receptors and potassium channels and shown to confer interactions with a family of synaptic proteins that promote ion channel clustering.

Function:
EAAT4 is expressed predominantly in the cerebellum (Purkinje cells) and weakly in the forebrain, where it transports L-glutamate, L-aspartate and D-aspartate, acting as a symport by cotransporting sodium. EAAT4 may function as a combined transporter and inhibitory glutamate receptor and also functions as an anion-selective ion channel.

Subcellular Location:
Membrane; multi-pass membrane protein.

Tissue Specificity:
Brain. Expressed densely and selectively incell bodies of Purkinje cells.

Similarity:
Belongs to the sodium:dicarboxylate (SDF) symporter(TC 2.A.23) family. SLC1A6 subfamily.

Database links:
UniProtKB/Swiss-Prot: P48664.1

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.