中文名稱    真核翻譯起始因子2C1抗體    

英文名稱    EIF2C    

別    名    Argonaute 1; Argonaute-1, isoform A; dAgo1; EIF 2C 1; EIF2C 1; EIF2C; EIF2C1; Eukaryotic translation initiation factor 2C 1; GERP95; Putative RNA binding protein Q99; Putative RNA-binding protein Q99; Q99; AGO1_HUMAN.    

供 應 商    遠慕生物      

研究領域    細胞生物  染色質和核信號  信號轉導  細胞周期蛋白  表觀遺傳學      

抗體來源    Rabbit    

克隆類型    Polyclonal    

交叉反應    Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Fruit Fly,     

產品應用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 真核翻譯起始因子2C1抗體（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    94kDa    

細胞定位    細胞漿     

性    狀    Lyophilized or Liquid    

濃    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human EIF2C1/Ago1    

亞    型    IgG    

純化方法    affinity purified by Protein A    

儲 存 液    Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4    

保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

真核翻譯起始因子2C1抗體產品介紹    background:

Ago1 belongs to the argonaute family and contains 1 PAZ and 1 Piwi domain. It is required for G1 arrest and mating in response to nitrogen starvation. It is also known as cell cycle control protein through it's regulation of cytokinesis and cell cycle checkpoints occurs downstream of dcr1. It is required, indirectly, for regulated hyperphosphorylation of cdc2. Ago 1 also has a role in the RNA interference (RNAi) pathway which is important for heterochromatin formation and accurate chromosome segregation. It has been shown to be non-essential for siRNA-directed target RNA cleavage but is needed for the production of mature miRNA that affects miRNA-directed RNA cleavage.

Function:
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) or short interfering RNAs (siRNAs), and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for transcriptional gene silencing (TGS) of promoter regions which are complementary to bound short antigene RNAs (agRNAs).

Subunit:
Interacts with DDB1, DDX5, DDX6, DHX30, DHX36, DDX47, DICER1, EIF2C2/AGO2, ELAVL1, HNRNPF, IGF2BP1, ILF3, IMP8, MATR3, MOV10, PABPC1, PRMT5, RBM4, SART3, TNRC6B, UPF1 and YBX1. Associates with polysomes and messenger ribonucleoproteins (mNRPs). Interacts with LIMD1, WTIP and AJUBA.

Subcellular Location:
Cytoplasm, P-body.

Similarity:
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain.

Database links:
UniProtKB/Swiss-Prot: Q9UL18.3 human
NCBI Reference Sequence: NP_725341.1 fruit fly

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.