英文名稱    ENO3    

中文名稱    骨骼肌烯醇化酶抗體    

別    名    2 phospho D glycerate hydrol yase; 2 phospho D glycerate hydrolyase; Beta enolase; ENO3; Enolase 3 (beta muscle); Enolase 3; Enolase3; GSD13; MSE; Muscle specific enolase; Skeletal muscle enolase; ENOB_HUMAN.    

供 應 商    遠慕生物

研究領域    腫瘤  細胞生物  免疫學      

抗體來源    Rabbit    

克隆類型    Polyclonal    

交叉反應    Human, Mouse, Rat, Chicken, Pig, Cow, Horse, Sheep,     

產品應用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 骨骼肌烯醇化酶抗體（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    47kDa    

性    狀    Lyophilized or Liquid    

濃    度    1mg/1ml    

免 疫 原    KLH conjugated synthetic peptide derived from human ENO3    

亞    型    IgG    

純化方法    affinity purified by Protein A    

儲 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

骨骼肌烯醇化酶抗體產品介紹    background:

ENO3 is also known as beta enolase, one of the three enolase isoenzymes found in mammals. A switch from alpha enolase to beta enolase occurs in muscle tissue during development and ENO3, a homodimer, is found in skeletal muscle cells in the adult and appears to have a function in striated muscle development and regeneration. Mutations can result in decreased stability of the enzyme and be associated with a glycogen storage myopathy. This results in exercise-induced myalgias, generalized muscle weakness and fatigability.

Function:
Appears to have a function in striated muscle development and regeneration.

Subunit:
Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. Interacts with PNKD.

Subcellular Location:
Cytoplasm. Note=Localized to the Z line. Some colocalization with CKM at M-band (By similarity).

Tissue Specificity:
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.

DISEASE:
Glycogen storage disease 13 (GSD13) [MIM:612932]: A metabolic disorder that results in exercise-induced myalgias, generalized muscle weakness and fatigability. It is characterized by increased serum creatine kinase and decreased enolase 3 activity. Dramatically reduced protein levels with focal sarcoplasmic accumulation of glycogen-beta particles are detected on ultrastructural analysis. Note=The disease is caused by mutations affecting the gene represented in this entry.

Similarity:
Belongs to the enolase family.

Database links:
UniProtKB/Swiss-Prot: P13929.5

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.