中文名稱    磷酸化真核翻譯起始因子4B抗體    

英文名稱    phospho-EIF4B (Ser422)    

別    名    EIF 4B; EIF-4B; Eukaryotic initiation factor 4B; IF4B_HUMAN.    

供 應 商    遠慕生物      

產品類型    磷酸化抗體     

研究領域    腫瘤  免疫學  細胞凋亡  轉錄調節因子      

抗體來源    Rabbit    

克隆類型    Polyclonal    

交叉反應    Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, Guinea Pig,     

產品應用    WB=1:100-500 ELISA=1:500-1000 IP=1:20-100 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 磷酸化真核翻譯起始因子4B抗體（石蠟切片需做抗原修復）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.    

分 子 量    69kDa    

性    狀    Lyophilized or Liquid    

濃    度    1mg/1ml    

免 疫 原    KLH conjugated Synthesised phosphopeptide derived from human EIF4B around the phosphorylation site of Ser422    

亞    型    IgG    

純化方法    affinity purified by Protein A    

儲 存 液    0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide    

保存條件    Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.    

磷酸化真核翻譯起始因子4B抗體產品介紹    background:

eIF4B is required for the binding of mRNA to ribosomes. It functions in close association with eIF4F and eIF4A. It binds near the 5'-terminal cap of mRNA in the presence of eIF4F and ATP. It promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both eIF4A and eIF4F (referenced from swissprot).

Function:
Required for the binding of mRNA to ribosomes. Functions in close association with EIF4-F and EIF4-A. Binds near the 5'-terminal cap of mRNA in presence of EIF-4F and ATP. Promotes the ATPase activity and the ATP-dependent RNA unwinding activity of both EIF4-A and EIF4-F.

Subunit:
Self-associates and interacts with EIF3 p170 subunit.

Post-translational modifications:
Phosphorylated at Ser-422 by RPS6KA1 and RPS6KB1; phosphorylation enhances the affinity of EIF4B for the EIF3 complex.

Similarity:
Contains 1 RRM (RNA recognition motif) domain.

Database links:
UniProtKB/Swiss-Prot: P23588.2

Important Note:
This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.